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A sample port is necessary for introducing the sample at the head of the column. modern injection techniques often employ the use of heated sample ports through which the sample can be injected and vaporized in a near simultaneous fashion. a calibrated microsyringe is used to deliver a sample volume in the range of a few microliters through a rubber septum and into the vaporization chamber. most separations require only a small fraction of the initial sample volume and a sample splitter is used to direct excess sample to waste. Commercial gas chromatographs often allow for both split and splitless injections when alternating between packed columns and capillary columns. the vaporization chamber is typically heated 50 °c above the lowest boiling point of the sample and subsequently mixed with the carrier gas to transport the sample into the column.

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