ApplicationMeasuring Light Absorption In Sample Solutions,Laboratory
Measurement TypeAbsorbance, Transmittance
Wavelength Range190-1100 Nm
Accuracy±1 Nm
A spectrophotometer is an instrument that measures the amount of photons (the intensity of light) absorbed after it passes through sample solution. With the spectrophotometer, the amount of a known chemical substance (concentrations) can also be determined by measuring the intensity of light detected.
The spectrophotometer measures the amount of visible light absorbed by a solution. To obtain a reading, the spectrophotometer will direct a known amount of light through a cuvette filled with solution and will measure the amount of light that passes through the cuvette.
Spectrophotometer specifications define its performance capabilities and components, including wavelength range (e.g., 190–1100 nm for UV-Vis), photometric range (Absorbance: -4 to +4.0 Abs), photometric accuracy (e.g., ±0.0015 Abs), wavelength accuracy (e.g., ±0.05 nm), spectral bandwidth (e.g., 1 nm or better), light source (tungsten and deuterium lamps), optics (e.g., double-beam, holographic grating), detector (e.g., silicon photodiode), and data connectivity (USB ports). Other key specs involve stray light, noise level, baseline stability, and on-board features like LCD displays, software, and hardware validation.
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