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Other Details : Thermo Scientific Phusion Site-Directed Mutagenesis Kit is a versatile and efficient tool for introducing point mutations, insertions, or deletions in any type of plasmid DNA With this kit, the entire plasmid is amplified using phosphorylated primers that introduce the desired changes The amplified, linear PCR product, containing the desired mutation, is circularized in a 5-minute ligation reaction with T4 DNA Ligase The resulting plasmid can be then transformed into any competent The optimal annealing temperature for Phusion DNA Polymerases may differ significantly from that of Taq-based polymerases Features : Excellent quality Air tight packaging Purity Cell Viability Assays : We are remarkable entity, engaged in offering superior quality Cell Viability Assays.