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Chronic toxicity studies are long-term investigations conducted to evaluate the health effects of a substance after prolonged or lifetime exposure. Typically lasting from 6 months to 2 years, depending on the test species, these studies are essential for understanding the potential risks associated with continuous exposure to chemicals, pharmaceuticals, or environmental agents. They help identify: Cumulative toxicity – effects that build up in the body over time. Target organs – specific organs that may be most affected by the substance. Carcinogenicity potential – the likelihood of the substance causing cancer. Safe exposure limits – thresholds below which the substance is considered safe for humans.   Chronic toxicity studies play a vital role in regulatory decision-making, supporting the development of safety guidelines and risk assessments to protect public health. They are especially important for substances used in food, medicine, and occupational settings.